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Choline dehydrogenase (CHDH) mediates c‐Jun activation to promote colorectal cancer (CRC) cell migration in vitro. (A, B) Activation of 39 kinases or key proteins were detected in RKO cells overexpressing CHDH or MCS using a human <t>phosphokinase</t> array. (C) Western blot analysis the expression of c‐Jun and p‐c‐Jun in CHDH knockdown or overexpression cells. (D, E) Representative immunohistochemistry (IHC) staining of CHDH and p‐c‐Jun in human CRC tissues and the correlation results were shown, scale bar: 50 µm. (F) Western blot analysis the expression of c‐Jun and p‐c‐Jun in CHDH overexpression cells treated with SP600125. (G) Wound‐healing assay images of RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 were shown. Scale bar: 50 µm. (H) Transwell assay images of RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 were shown. Scale bar: 50 µm. (I) Statistic results on wound closure in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125. ** p < 0.01, *** p < 0.001. (J) Statistic results on migrated cells in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125. *** p < 0.001. (K) Immunofluorescence assay to detect F‐actin rearrangement in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 via phalloidin staining. Scale bar: 20 µm. (L) Western blot analysis the expression of epithelial–mesenchymal transition (EMT) markers in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125.
Human Phosphokinase Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human phosphokinase array kit/product/R&D Systems
Average 96 stars, based on 1 article reviews
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Choline dehydrogenase (CHDH) mediates c‐Jun activation to promote colorectal cancer (CRC) cell migration in vitro. (A, B) Activation of 39 kinases or key proteins were detected in RKO cells overexpressing CHDH or MCS using a human <t>phosphokinase</t> array. (C) Western blot analysis the expression of c‐Jun and p‐c‐Jun in CHDH knockdown or overexpression cells. (D, E) Representative immunohistochemistry (IHC) staining of CHDH and p‐c‐Jun in human CRC tissues and the correlation results were shown, scale bar: 50 µm. (F) Western blot analysis the expression of c‐Jun and p‐c‐Jun in CHDH overexpression cells treated with SP600125. (G) Wound‐healing assay images of RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 were shown. Scale bar: 50 µm. (H) Transwell assay images of RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 were shown. Scale bar: 50 µm. (I) Statistic results on wound closure in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125. ** p < 0.01, *** p < 0.001. (J) Statistic results on migrated cells in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125. *** p < 0.001. (K) Immunofluorescence assay to detect F‐actin rearrangement in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 via phalloidin staining. Scale bar: 20 µm. (L) Western blot analysis the expression of epithelial–mesenchymal transition (EMT) markers in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125.
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Choline dehydrogenase (CHDH) mediates c‐Jun activation to promote colorectal cancer (CRC) cell migration in vitro. (A, B) Activation of 39 kinases or key proteins were detected in RKO cells overexpressing CHDH or MCS using a human <t>phosphokinase</t> array. (C) Western blot analysis the expression of c‐Jun and p‐c‐Jun in CHDH knockdown or overexpression cells. (D, E) Representative immunohistochemistry (IHC) staining of CHDH and p‐c‐Jun in human CRC tissues and the correlation results were shown, scale bar: 50 µm. (F) Western blot analysis the expression of c‐Jun and p‐c‐Jun in CHDH overexpression cells treated with SP600125. (G) Wound‐healing assay images of RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 were shown. Scale bar: 50 µm. (H) Transwell assay images of RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 were shown. Scale bar: 50 µm. (I) Statistic results on wound closure in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125. ** p < 0.01, *** p < 0.001. (J) Statistic results on migrated cells in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125. *** p < 0.001. (K) Immunofluorescence assay to detect F‐actin rearrangement in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 via phalloidin staining. Scale bar: 20 µm. (L) Western blot analysis the expression of epithelial–mesenchymal transition (EMT) markers in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125.
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proteome profiler human phospho kinase array kit  (R&D Systems)
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Choline dehydrogenase (CHDH) mediates c‐Jun activation to promote colorectal cancer (CRC) cell migration in vitro. (A, B) Activation of 39 kinases or key proteins were detected in RKO cells overexpressing CHDH or MCS using a human <t>phosphokinase</t> array. (C) Western blot analysis the expression of c‐Jun and p‐c‐Jun in CHDH knockdown or overexpression cells. (D, E) Representative immunohistochemistry (IHC) staining of CHDH and p‐c‐Jun in human CRC tissues and the correlation results were shown, scale bar: 50 µm. (F) Western blot analysis the expression of c‐Jun and p‐c‐Jun in CHDH overexpression cells treated with SP600125. (G) Wound‐healing assay images of RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 were shown. Scale bar: 50 µm. (H) Transwell assay images of RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 were shown. Scale bar: 50 µm. (I) Statistic results on wound closure in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125. ** p < 0.01, *** p < 0.001. (J) Statistic results on migrated cells in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125. *** p < 0.001. (K) Immunofluorescence assay to detect F‐actin rearrangement in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 via phalloidin staining. Scale bar: 20 µm. (L) Western blot analysis the expression of epithelial–mesenchymal transition (EMT) markers in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125.
Proteome Profiler Human Phospho Kinase Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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proteome profiler human phospho kinase array kit - by Bioz Stars, 2026-03
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Choline dehydrogenase (CHDH) mediates c‐Jun activation to promote colorectal cancer (CRC) cell migration in vitro. (A, B) Activation of 39 kinases or key proteins were detected in RKO cells overexpressing CHDH or MCS using a human phosphokinase array. (C) Western blot analysis the expression of c‐Jun and p‐c‐Jun in CHDH knockdown or overexpression cells. (D, E) Representative immunohistochemistry (IHC) staining of CHDH and p‐c‐Jun in human CRC tissues and the correlation results were shown, scale bar: 50 µm. (F) Western blot analysis the expression of c‐Jun and p‐c‐Jun in CHDH overexpression cells treated with SP600125. (G) Wound‐healing assay images of RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 were shown. Scale bar: 50 µm. (H) Transwell assay images of RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 were shown. Scale bar: 50 µm. (I) Statistic results on wound closure in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125. ** p < 0.01, *** p < 0.001. (J) Statistic results on migrated cells in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125. *** p < 0.001. (K) Immunofluorescence assay to detect F‐actin rearrangement in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 via phalloidin staining. Scale bar: 20 µm. (L) Western blot analysis the expression of epithelial–mesenchymal transition (EMT) markers in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125.

Journal: MedComm

Article Title: Prolyl 4‐hydroxylase α‐subunit family regulation of type I collagen deposition and IL17RB/c‐Jun activation synergistically mediate choline dehydrogenase promotion of colorectal cancer metastasis

doi: 10.1002/mco2.70007

Figure Lengend Snippet: Choline dehydrogenase (CHDH) mediates c‐Jun activation to promote colorectal cancer (CRC) cell migration in vitro. (A, B) Activation of 39 kinases or key proteins were detected in RKO cells overexpressing CHDH or MCS using a human phosphokinase array. (C) Western blot analysis the expression of c‐Jun and p‐c‐Jun in CHDH knockdown or overexpression cells. (D, E) Representative immunohistochemistry (IHC) staining of CHDH and p‐c‐Jun in human CRC tissues and the correlation results were shown, scale bar: 50 µm. (F) Western blot analysis the expression of c‐Jun and p‐c‐Jun in CHDH overexpression cells treated with SP600125. (G) Wound‐healing assay images of RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 were shown. Scale bar: 50 µm. (H) Transwell assay images of RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 were shown. Scale bar: 50 µm. (I) Statistic results on wound closure in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125. ** p < 0.01, *** p < 0.001. (J) Statistic results on migrated cells in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125. *** p < 0.001. (K) Immunofluorescence assay to detect F‐actin rearrangement in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125 via phalloidin staining. Scale bar: 20 µm. (L) Western blot analysis the expression of epithelial–mesenchymal transition (EMT) markers in RKO and LOVO expressing CHDH cells treated with DMSO (Ctrl) or SP600125.

Article Snippet: The Human Phosphokinase Array Kit was purchased from R&D Systems (Cat. #ARY003C), then was used to quantify relative kinase and protein phosphorylation levels following the manufacturer's instructions.,

Techniques: Activation Assay, Migration, In Vitro, Western Blot, Expressing, Knockdown, Over Expression, Immunohistochemistry, Wound Healing Assay, Transwell Assay, Immunofluorescence, Staining